多克隆抗體定制服務

       多克隆抗體能夠識別多個抗原表位，并且由于制備周期短，成本低，應用廣泛等優點已成為科研人員進 行免疫學研究不可或缺的重要工具。天正源生物憑借5000+多抗項目研發和生產經驗，可以提供兔、大鼠、小鼠、豚鼠、 羊、雞等多個宿主在內的多抗制備。并且已成功定制過以下多種屬蛋白質抗體：人、小鼠、大鼠、斑馬魚、銀鯽、草魚、 黃顙魚、鱖魚、海參、海膽、線蟲、、四膜蟲、細菌、病毒、擬南芥、藍藻、棉花、玉米、水稻、馬鈴薯、油菜、小麥等，實戰經驗非常豐富，提供多個物種，多種抗原，多個應用平臺的多克隆抗體定制，能夠最大程度保證抗體定制的成功率。

• 免費分析抗原序列，利用專業的抗原設計軟件設計抗原，結合文獻，綜合選擇免疫原序列
• 提供優質重組蛋白質抗原（原核+真核），用于免疫的抗原（上清表達成功率>90%），純度>80%，濃度 >1mg/ml
• 合成高純度多肽抗原，提供多肽質檢報告；小分子抗原采用獨特的活化和偶聯方案
• 利用自主研發佐劑，保證抗原和佐劑充分混勻，多點皮下注射，最大程度上提高動物的血清效價
• 構建第二套載體表達蛋白，用于純化血清，減少標簽蛋白對抗體特異性的影響，純化出高特異性抗體

• 客戶進行Western Blot內源性或者IP過表達檢測小樣后能夠檢測到目的條帶（說明抗體驗證合格）；
• Western Blot內源性或者IP過表達檢測陰性無法滿足實驗需要，說明此抗體訂單驗證失敗，則甲方無需再付此項目剩余尾款；
• 間接ELISA檢測抗血清效價，針對免疫原效價，兔多抗不低于1:160,000，小鼠多抗不低于1:6,4000；
• 客戶有權隨時了解服務項目進展情況，并對成果擁有全部所有權。

[1] Xiaobo Yu,et al. Fertilized egg cells secrete endopeptidases to avoid polytubey. Nature. 31 March.2021

[2] Zhaojing Lu ,et al. Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration .JBC. July 30, 2019   

[3] Meng-Jie Zhao,et al. MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation [J]. Cell Death & Disease. 22 February 2019

[4] Miao Li1,2,3*, Tao Huang1,2,3*, Meng-Jing Li1,2,3*, et al. The histone modification reader   ZCWPW1 is requiredfor meiosis prophase I in male but not in female mice [J].

Science Advances 14 Aug 2019: Vol. 5, no. 8, eaax1101

[5] Functional characterization of IL-10 and its receptor subunits in a perciform fish, the mandarin fish, Siniperca chuatsi [J]. Developmental & Comparative Immunology.

Volume 97, August 2019, Pages 64-75

[6] Yao Wang1 ,et al. Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS [J].Nature Cell Biology

[7] Linlin Shi 1, Weimin Wang 1 and Yanhe Li 1,4,*et al. Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene   in the Red Swamp Crayfish, Procambarus clarkii[J]. MDPI Genes 2019

[8] Jing Hou, Shan Nan Chen, Zhen Gan, Nan Li, Lin Huang, Hui Jun Huo, Yue Cong Yang, Yishan Lu, Zhan Yin and Pin Nie   In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN-γ, IRF1, and Two Forms of CIITA

[9]JunyanTao, Chenglian Bai, Yuanhong Chen, Huanhuan Zhou, Yahui Liu, Qingyu Shi, WenhaoPan, Haojia Dong, Luyi Li, Hui Xu, Robyn Tanguay, Changjiang Huang, QiaoxiangDong   Environmental relevant concentrations of benzophenone-3 induced developmental neurotoxicity in zebrafish[J].Science of The Total Environment 2020

[10]Qiannan Zhang1 , Yanghong Xu1, Jishuai Huang1, Kai Zhang2, Haijun Xiao3 8 ,XiaojianQin4, Linlin Zhu5, Yingguo Zhu1,#, and Jun Hu1*   Therice pentatricopeptide repeat protein PPR756 is involved in pollen developmentby affecting multiple RNA editing in mitochondria.12 Jun 2020

[11]Qiannan Zhang1 , Yanghong Xu1, Jishuai Huang1, Kai Zhang2, Haijun Xiao3 8 ,XiaojianQin4, Linlin Zhu5, Yingguo Zhu1,#, and Jun Hu1*   The rice PPR756 coordinates with MORFsfor multiple RNA editing in mitochondria.2019